Purpose: Obtain a venous blood sample for laboratory analysis.
- Select vein for venipuncture (usually antecubital space) *
- Apply tourniquet several inches above intended puncture site
- Clean venipuncture site (with povidone-iodine or alcohol); allow area to dry
- Perform venipuncture by entering the skin with needle at approximately a 15-degree angle to the skin, needle bevel up
- If using a vacutainer, ease tube forward in holder once in the vein. If using a syringe, pull back on the barrel with slow, even tension as blood fills the syringe.
- Release tourniquet with the blood begins to flow
- After blood is drawn, place cotton ball over site; withdraw the needle and eert pressure. Apply bandage if needed.
- Mix additive tubes with gentle rolling motion (no not vigorously shake tubes). If specimens collected in syringe, transfer to appropriate tubes
- Properly dispose of contaminated materials
- Record the date and time of blood collection. Attach a label to each blood tube.
Note: Do not use a vein site proximal to an IV infusion.
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